Expression of Pin1 in malignant hematopoietic cells and its relation with cell cycle.
- Author:
Yuan-yuan ZHU
1
;
Ji-min SHI
;
Jie SUN
;
Jian-ping LAN
;
Xiao-yu LAI
;
Jing-yuan LI
;
Jian YU
;
Ya-min TAN
;
Mao-fang LIN
;
He HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; physiology; G1 Phase; Humans; Leukemia, Lymphoid; enzymology; pathology; Leukemia, Myeloid; enzymology; pathology; Peptidylprolyl Isomerase; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; S Phase; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2004;33(6):500-514
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of peptidyl-prolyl cis/trans isomerase (PPIase or Pin1) in malignant hematopoietic cells and its relation with cell cycle.
METHODSRealtime quantitative PCR with fluorescence probe hybridization was used to measure expression of Pin1 mRNA in malignant hematopoietic cell lines and normal mononuclear cells separated from bone marrow. HeLa cells were blocked with Thymidine and Nocodazole in different cell phases and then the expression of Pin1 mRNA and protein were detected by realtime-PCR and immunoblotting.
RESULTSThe expression of Pin1 in malignant hematopoietic cell lines was significantly higher than that in normal controls (0.339 +/-0.093 compared with 0.038 +/-0.005, P<0.01). Its expression in myeloid malignant hematopoietic cell lines was significantly higher than that in normal controls (0.388 +/-0.115 compared with 0.038 +/-0.005, P<0.01) and so was the malignant lymphocytic cell lines (0.226 +/-0.166 compared with 0.038 +/-0.005, P<0.01). The expression of Pin1 was closely correlated with cell cycle. It was the highest in G1 phase and the lowest in S phase (110.762 +/-16.737 compared with 4.080 +/-0.634, P<0.01).
CONCLUSIONPin1 is overexpressed in malignant hematopoietic cell lines and its expression is different during cell cycle that is highest in G1 phase and lowest in S phase.
