Establishment of computer-based video-tracking conditioned place preference experiment system in mice.
- Author:
Zai-hao LONG
1
;
Yong-ping ZHU
;
Hui-hong CHEN
;
Lian-fang ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Automatic Data Processing; Conditioning (Psychology); drug effects; physiology; Male; Mice; Mice, Inbred ICR; Models, Animal; Morphine; pharmacology; Motor Activity; drug effects; Narcotics; pharmacology; Opioid-Related Disorders; physiopathology; psychology; Video Recording
- From: Journal of Zhejiang University. Medical sciences 2004;33(6):524-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the computer-based video-tracking conditioned place preference (CPP) system in mice.
METHODSThe CPP system was composed of computer, camera, soundproof box, shuttle box and analytical software. The results of morphine-induced conditioned place preference were used to evaluate the experiment system. And the effect of morphine-induced locomotor activity in drug-paired compartment was studied in mice.
RESULTSLow (1 mg/kg, i.p.), moderate (3 mg/kg, 5 mg/kg, i.p.) and high (10 mg/kg, i.p.) dose of morphine significantly prolonged the time mice spent in drug-paired compartment compared with saline, but there was no dose-response relation. Moderate and high dose of morphine significantly enhanced locomotor activity, among which 5 mg/kg and 10 mg/kg morphine induced behavior sensitization in drug-paired compartment during the conditioning sessions.
CONCLUSIONThe computer-based video-tracking conditioned place preference experiment system in mice established successfully is reliable and stable.
