Effect of HIV Tat protein on CCR5 expression in monocytes and infection with monocyte-tropic HIV strains.
- Author:
Yi-da YANG
1
;
Lin ZHENG
;
Guo-cai LU
;
Ya-gang CHEN
;
Maria SALVATO
Author Information
- Publication Type:Journal Article
- MeSH: Flow Cytometry; Gene Products, tat; pharmacology; HIV; HIV Infections; metabolism; Humans; Monocytes; metabolism; Receptors, CCR5; biosynthesis; genetics; tat Gene Products, Human Immunodeficiency Virus
- From: Journal of Zhejiang University. Medical sciences 2004;33(6):532-545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of HIV Tat protein on CCR5 expression of monocytes and HIV infection in monocytes.
METHODSMembrane expression of CCR5 on monocytes was analyzed by flow cytometry. Stimulated with HIV Tat protein, monocytes were infected with monocyte-tropic HIV(Ba-L) and HIV gag p24 level in the supernatant was measured by ELISA methods.
RESULTSHIV Tat protein increased CCR5 expression in human monocytes,which was inhibited by rabbit anti-Tat polyclonal antibody. Tat protein also increased p24 level after monocyte-tropic HIV-1(Ba-L) infected monocytes.
CONCLUSIONTat increases CCR5 expression and HIV-1 infection in monocytes, which indicates that HIV Tat might be a key protein in HIV-1 infection.
