Suppression of Tacrolimus on the expression of MMP-9 in rhHGF-stimulated ECV304 cell line.
- Author:
Yang LIU
1
;
Min ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; drug effects; Cell Line; Endothelial Cells; cytology; metabolism; Flow Cytometry; Hepatocyte Growth Factor; pharmacology; Humans; Immunosuppressive Agents; pharmacology; Matrix Metalloproteinase 9; biosynthesis; Recombinant Proteins; pharmacology; Skin; metabolism; Tacrolimus; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2004;33(6):540-545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the stimulating effect of recombinant human hepatocyte growth factor (rhHGF) on the expression of matrix metalloproteinase-9 (MMP-9) in ECV304 cells and the suppression effect of FK506.
METHODSThe growth curve of ECV304 cells was drawn and the valid concentration of rhHGF and FK506 were determined. The expression levels of MMP-9 were analyzed by flow cytometry (FCM).
RESULTS(1) The optimal growth concentration of ECV304 cell was 1.0E+4/ml and logarithm growth time was 3-5 days. The valid concentration of rhHGF was 8 ng/ml and IC50 was 8.27 ng/ml. The valid concentration of FK506 was 50 ng/ml and IC50 was 51.63 ng/ml. (2) The expression level of MMP-9 was 39.74% in controls. Cell livability was 1.388169 +/-0.102033 (P<0.01) when 8 ng/ml rhHGF was added to culture medium, and the expression level of MMP-9 was 40.32%. Cell livability was 0.398764 +/-0.092476 (P<0.01) when 50 ng/ml FK506 was added to culture medium, and the expression level of MMP-9 was 14.61%. Cell livability was 0.767203 +/-0.02639 (P<0.01) when 8 ng/ml rhHGF was added 15 minutes after 50 ng/ml FK506 was added to culture medium, and the expression level of MMP-9 was 35.08%.
CONCLUSIONrhHGF can stimulate the proliferation of ECV304 cells and increase the protein expression level of MMP-9. FK506 can suppress the proliferation of ECV304 cells and decrease the expression level of MMP-9. FK506 can inhibit the proliferation of ECV304 cells induced by rhHGF and decrease MMP-9 protein level.
