Establishment of two-dimensional electrophoresis of uterine leiomyoma for the proteome analysis.

Author: Xue-qiong ZHU ¹ ; Yi-fu SHI ; Huai-zeng CHEN ; Qi CHEN
Author Information

1. Women's Hospital, College of Medicine, Zhejiang University, Hangzhou, 310006, China.
Publication Type:Journal Article
MeSH: Electrophoresis, Gel, Two-Dimensional; Female; Humans; Leiomyoma; chemistry; Myometrium; chemistry; Neoplasm Proteins; analysis; Proteome; analysis; Uterine Neoplasms; chemistry
From: Journal of Zhejiang University. Medical sciences 2004;33(6):550-564
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo establish and optimize the two-dimensional electrophoresis (2-DE) of uterine leiomyoma for the proteome analysis.
METHODSRun immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.
RESULTSThe 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.
CONCLUSIONWith optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.