Study on post-translational processing and active forms of the novel metastasis-associated protein SNC19.
- Author:
Ying SHI
1
;
Shu ZHENG
;
Xiao-Ye HU
;
Li-feng SUN
;
Su-zhan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; genetics; metabolism; pathology; Cell Line, Tumor; Colonic Neoplasms; genetics; metabolism; pathology; DNA, Complementary; chemistry; Female; Gelatinases; metabolism; Humans; Neoplasm Metastasis; Neoplasm Proteins; chemistry; genetics; metabolism; Protein Processing, Post-Translational; Serine Endopeptidases; chemistry; genetics; metabolism; Transfection
- From: Journal of Zhejiang University. Medical sciences 2005;34(1):38-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between SNC19 protein and cancer metastasis.
METHODSExpression of SNC19 protein in cancer cell lines and tissues was examined by Western blot analysis using anti-SNC19 monoclonal antibody. In addition, Psectag2A-SNC19(ORF) eukaryotic expression vector was constructed and transfected into BCAP37 cells. After the target protein was expressed and purified, processing forms of SNC19 protein were further identified using anti-His mAb and each form was assayed for its gelatinase activity.
RESULTSDifferent expression and post-translational processing of the SNC19 proteins in the cancer cell lines and intestinal tissues were detected.BCAP37 cells transfected full-length SNC19 (ORF) generated two different sized proteins in cell lysates, 120 and 75 kD; 75 kD was detected to have proteolytic activity by gelatin zymography.
CONCLUSIONSNC19 protein presents different expression and post-translational processing in the cancer cells and tissues, of which 75 kD was identified to have gelatinase activity.
