Therapeutic effect of cationic liposome-mediated interleukin-12 gene delivery on murine melanoma in vivo.
- Author:
Wei CHEN
1
;
Chuan-sen SHAO
;
Jian-gen SHEN
;
Yi PAN
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cations; DNA; therapeutic use; Female; Interleukin-12; genetics; therapeutic use; Killer Cells, Natural; immunology; Liposomes; Melanoma, Experimental; pathology; therapy; Mice; Mice, Inbred C57BL; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2005;34(1):73-76
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the therapeutic effect of cationic liposome-mediated interleukin-12 gene delivery on established murine melanoma in vivo.
METHODSThe lipofectin encapsulated pCmIL-12 plasmid was given to C57BL/6 mice on the day 3,5,7,9 after inoculation of B16 melanoma cells. The tumor size, the survival time of mice and the NK cell activity were observed.
RESULTSThe pCmIL-12 plasmid coupled with cationic liposome inhibited the tumor growth and improved the survival of mice bearing established melanoma. The activity of NK cells was also enhanced after interleukin-12 gene delivery in vivo.
CONCLUSIONCationic liposome-mediated interleukin-12 gene delivery has significantly therapeutic effects on mice melanoma in vivo.
