Inhibition of binding peptides on replication of duck hepatitis B virus.
- Author:
Hong-yu JIA
1
;
Zhi CHEN
;
Lin-fu ZHOU
;
Feng CHEN
;
Hai-hong ZHU
;
Ji-hong LIU
;
Xiao-yan XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Ducks; Hepadnaviridae Infections; virology; Hepatitis B Virus, Duck; drug effects; genetics; Hepatitis, Viral, Animal; virology; Hepatocytes; virology; Peptide Nucleic Acids; pharmacology; RNA-Directed DNA Polymerase; metabolism; Virus Replication; drug effects
- From: Journal of Zhejiang University. Medical sciences 2005;34(2):116-120
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effect of binding peptides on duck hepatitis B virus (DHBV) replication in duck hepatocytes.
METHODSSpecific binding peptides to duck hepatitis B virus polymerase (DHBVP) were screened by phage display technology (PDT), then were sequenced and synthesized. Binding peptides were added into primary culture of duck hepatocytes infected with DHBV in vitro. DHBV-DNA in the cytoplasm, cell nucleus and medium supernatant was assayed over time.
RESULTSSeven binding peptides were obtained after 3-round screening by PDT. Duck primary hepatocytes infected by DHBV were treated with above obtained binding peptides. The DHBV-DNA levels in medium supernatant and cytoplasm of duck hepatocytes treated with synthesized peptides (the 3rd and the 6th peptide) were significantly lower than those of control cells (P<0.05).
CONCLUSIONSpecific binding peptides to DHBVP could inhibit the replication of DHBV.
