Expression of alpha-smooth muscle actin in kidney of diabetic rats.
- Author:
Hong LI
1
;
Zhe ZHANG
;
Hong-Lei WEN
;
Yu RUAN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; biosynthesis; genetics; Animals; Biomarkers; Collagen Type IV; biosynthesis; genetics; Diabetes Mellitus, Experimental; metabolism; Diabetic Nephropathies; metabolism; Enzyme-Linked Immunosorbent Assay; Female; Kidney; metabolism; Male; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2005;34(2):152-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of alpha smooth muscle actin (alphaSMA), a marker of myofibroblast in kidney of diabetic rats and its role in pathogenesis of diabetic nephropathy.
METHODSDiabetic disease model was made on 24 Sprague-Dauley (SD) rats by an single intraperitoneal injection of streptozotocin (70 mg/kg) and 24 health SD rats served as controls. Six diabetic rats and six control rats were sacrificed at 1, 2, 4, 8 weeks after injection of streptozotocin. At each time-point, the renal morphological changes were examined by histopathology. alpha SMA and collagen-IV (C-IV) expressions were studied by immunohistochemistry (SABC). The content of alpha SMA in renal cortex was detected by enzyme-linked immunosorbent assay(ELISA).
RESULTSAt all time-points, renal content of alpha SMA increased in diabetic rats. Compared with controls, diabetic rats had a steady increased accumulation of C-IV. Expression of C-IV was positively correlated with that of alpha SMA.
CONCLUSIONAt early stage of diabetes,increased alpha SMA in kidney suggests the formation of myofibroblast, which may be involved in accumulation of C-IV and contributed to diabetic nephropathy.
