Mapping of regulatory domain of T-protein from Escherichia coli.
- Author:
Hui ZHANG
1
;
Shu-qing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cloning, Molecular; Deoxyribonucleases, Type I Site-Specific; analysis; chemistry; genetics; Escherichia coli Proteins; analysis; chemistry; genetics; Nucleic Acid Conformation; Peptide Fragments; chemistry; genetics; Protein Binding; genetics; Regulatory Elements, Transcriptional; genetics
- From: Journal of Zhejiang University. Medical sciences 2005;34(2):181-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo map the regulatory domain of Escherichia coli T-protein.
METHODSFragmentation cloning was employed in cloning of 11 fragments from T-protein. The regulatory activity of each fragment was determined respectively.
RESULTSThe regulatory domain of T-protein was located in the C-terminal 270 amino acids, which was the same location as PDH domain.
CONCLUSIONT-protein has no independent regulatory domain.
