Gene expression of bone mesenchymal stem cells transduced by the lentiviral vector of SOX9 gene.
- Author:
Jie-yu BAI
1
;
Da-chuan LIANG
;
Peng CHENG
;
Zi-quan YANG
;
Xiao-chun WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Gene Expression; Genetic Therapy; Genetic Vectors; Lentivirus; genetics; Male; Mesenchymal Stromal Cells; metabolism; Mice; Osteoarthritis; therapy; Reverse Transcriptase Polymerase Chain Reaction; SOX9 Transcription Factor; genetics; Transduction, Genetic; Transfection
- From: China Journal of Orthopaedics and Traumatology 2013;26(7):597-600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct one lentiviral vector containing mouse SRY-related high mobility group-box gene 9 (SOX9) and transfect the murine bone mesenchymal stem cells (mBMSCs) in vitro and observe the expression of target gene.
METHODSRNA from the vectors containing mouse SOX9 gene were extracted and SOX9 genes were amplified by reverse transcription-Polymerase Chain Reaction (RT-PCR). The SOX9 genes were connected into lentiviral vectors pGC-FU. Then pGC-FU-SOX9 transduced into 293T cells to produce recombinant lentivirus called as Lenti-SOX9-EGFP. mBMSCs were transfected. The expression of target gene was detected by immunofluorescence, RT-PCR and Western Blot.
RESULTSLenti-SOX9-EGFP was recombined successfully and transduced efficiently into mBMSCs. The expression of SOX9 gene was confirmed by RT-PCR and Western Blot.
CONCLUSIONLentiviral vector of mouse SOX9 gene can transfect successfully into mBMSCs. Meanwhile, SOX9 gene may be expressed in mBMSCs. This will provide the target cells for the following study about SOX9 gene repairing cartilage injury.
