Gene expression of bone mesenehymal stem cells transduced by the lentiviral vector of SOX9 gene knockdown.
- Author:
Da-Chuan LIANG
1
;
Jie-Yu BAI
1
;
Shao-Hua DU
1
;
Peng CHENG
1
;
Zhen WANG
1
;
Ning KANG
1
;
Zi-Quan YANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Gene Expression; Gene Silencing; Genetic Therapy; Genetic Vectors; Lentivirus; genetics; Male; Mesenchymal Stromal Cells; metabolism; Mice; SOX9 Transcription Factor; genetics; Transduction, Genetic
- From: China Journal of Orthopaedics and Traumatology 2013;26(9):760-763
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct one lentiviral vector containing mouse SRY-related silencing group--box gene 9 (SOX9) and to transfect murine bone mesenehymal stem cells (mBMSCs) in vitro and observe the expression of target gene.
METHODSRNA inteference target sequence was designed in connectin with mice SOX9 gene sequence. The double strands DNAoligo containing interference sequence were synthesized and cloned into lentivirus vector. The siRNA lentiviral vector with SOX9 gene silencing was constructed and identified, which was transfected into rat bone mesenehymal stem cells. The expression of target gene was detected by immunofluorescence, RT-PCR and Western blot.
RESULTSLenti-SOX9-siRNA-EGFP was recombined successfully and transduced efficiently into mBMSCs. The expression of SOX9 gene silencing was confirmed by RT-PCR and Western blot.
CONCLUSIONMouse SOX9 gene silencing by RNA interference and Lentiviral vector can transfected successfully into mBMSCs. Meanwhile,SOX9 gene may be silenced in SOX9 transduced mBMSCs. This will provide target cells for the following study about SOX9 gene respairing cartilage injury.
