Growth of osteoblasts following culture on two collagen scaffolds.
- Author:
Chao MA
1
;
Ding ZHANG
;
Ping LI
;
Xiao-Jiao LI
Author Information
- Publication Type:Journal Article
- MeSH: Biocompatible Materials; Cell Adhesion; Cell Line; Cell Movement; Cell Proliferation; Collagen; Humans; Osteoblasts; cytology; ultrastructure; Tissue Scaffolds
- From: Acta Academiae Medicinae Sinicae 2011;33(5):538-542
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the growth of osteoblasts following culture on two absorbable collagen scaffolds.
METHODSMC3T3-E1 osteoblast cell line was inoculated on two collagen scaffolds (BME-10X and Bio-Gide) and co-cultured in vitro. The adhesion and migration of cells were detected by optical microscope. Cell proliferation was detected using CCK-8 reagent kit. The ultrastructure of the adhesion between cells and scaffolds were observed using electronic microscopy.
RESULTSMC3T3-E1 osteoblast cell line could adhere, migrate, and proliferate on both two membranes. The proliferation of cells showed no significant difference between porous layer and nonporous layer of BME-10X (P > 0.05), while the rate of proliferation was significantly different between loose layer and dense layer of Bio-Gide (P < 0.05).
CONCLUSIONBoth BME-10X and Bio-Gide have good biocompatibility with MC3T3-E1 osteoblast cell line, and the double-layer structure of Bio-Gide can prevent the cells to grow into the dense layer.
