Screening and identification of natural antisense transcript in mouse cerebral cortex.
- Author:
Ping LI
1
;
Jing ZHANG
;
Ai-Hua LI
;
Shan WANG
;
Xiao-Chao TAN
;
Bin YIN
;
Xiao-Zhong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cerebral Cortex; Mice; Molecular Sequence Data; RNA, Antisense; genetics; RNA, Messenger; genetics
- From: Acta Academiae Medicinae Sinicae 2011;33(6):620-623
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen and identify the possible existence of natural antisense transcript (NAT) within the mouse neocortex.
METHODSSixty-three cerebral cortex layer-specific genes were screened by bioinformatics prediction in mice, among which 31 mice with potential NATs were screened. NAT was identified using reverse transcription polymerase chain reaction (RT-PCR) and then cloned in pGEM-T Vector System for sequencing.
RESULTSAmong 31 genes predicted using bioinformatics, 8 were proved to be NAT positive by RT-PCR.
CONCLUSIONSNATs exist in the mouse neocortex tissue during the development of cerebral cortex. NATs may influence mouse cortical development by regulating the related coding genes.
