Separation and morphological observation of mouse testis seminiferous tubule epithelium.
- Author:
Jun FU
1
;
Shi-Ying MIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Separation; methods; Cells, Cultured; Male; Mice; Seminiferous Epithelium; cytology; Spermatozoa; cytology
- From: Acta Academiae Medicinae Sinicae 2011;33(6):629-631
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore a simple and feasible technique to locate proteins during spermatogenesis.
METHODSVarious germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy.
RESULTSGerm cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus.
CONCLUSIONA simple method for locating proteins during spermatogenesis was successfully developed.
