The in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1).
- Author:
Xing-quan ZHANG
1
;
Jun-peng DAI
;
Bai-qiang CAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antiviral Agents; pharmacology; Dogs; Indoles; pharmacology; Influenza A Virus, H1N1 Subtype; drug effects; Madin Darby Canine Kidney Cells; Virus Replication; drug effects
- From: Acta Academiae Medicinae Sinicae 2012;34(2):126-129
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1).
METHODSThe activity of arbidol hydrochloride against 2009 new influenza virus A (H1N1) was determined in MDCK cell cultures. Hemagglutination assay, observation of cytopathic effects, RT-PCR and quantitative RT-PCR tests were performed for determination of virus titers. Inhibition concentration 50% and cytotoxic concentration 50% were calculated with Chou's Menu of Dose-Effect Program.
RESULTSArbidol hydrochloride showed low cytotoxicity (cytotoxic concentration 50%>100 μmol/L)and significant anti-2009 new influenza virus A (H1N1) activity in cell cultures. Inhibition concentration 50% were (5.5 ± 0.9), (3.4 ± 0.8), and (1.5 ± 0.2) μmol/L in hemagglutination assay, cytopathic effect test, and quantitative RT-PCR assay, respectively.
CONCLUSIONArbidol has low cytotoxicity and high anti-virus activity and can effectively trigger the activities of interferon and immune response, and therefore can be a valuable anti-influenza virus drug.
