Expression of gene associated with retinoid-interferon-induced mortality-19 in preimplantation embryo of mice.
- Author:
Wen-juan CUI
1
;
Lan CHAO
;
Xiao-hui DENG
;
Yan-jun SHEN
;
Fang YANG
;
Wen-juan FENG
;
Jing XU
;
Hong-lei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blastocyst; drug effects; metabolism; Female; Interferons; pharmacology; Mice; NADH, NADPH Oxidoreductases; genetics; metabolism; Pregnancy; RNA, Messenger; genetics; Tretinoin; pharmacology
- From: Acta Academiae Medicinae Sinicae 2012;34(3):212-215
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the expression of gene associated with retinoid-interferon-induced mortality-19(GRIM-19) in preimplantation embryo of mice and explore its role in embryonic development.
METHODSThe protein and mRNA expressions of GRIM-19 in 2-cell, 4-cell, 8-cell, morula, and blastocyst phases of mice preimplantation embryo were detected by Western blot analysis and Real-time polymerase chain reaction (PCR).
RESULTSGRIM-19 was continuously expressed in every stage of preimplantation embryo of mice. Western blot analysis and Real-time PCR demonstrated a gradual increase of GRIM-19 expression from 2-cell, which reached a peak in 8-cell phase and then decreased progressively.
CONCLUSIONSThe expression of GRIM-19 in mouse preimplantation embryos changes as at different developmental phases. GRIM-19 may play an important role during embryonic development.
