Effect of LY294002 on adriamycin-induced epithelial-mesenchymal transition in human breast carcinoma cells.
- Author:
Yu-jun QIAN
1
;
Xiang-ming CHENG
;
Bin WANG
;
Lei XU
;
Chen-jiang WU
;
Meng-lin ZHAO
;
Xiao-hong QI
;
Jun GUO
Author Information
- Publication Type:Journal Article
- MeSH: Breast Neoplasms; metabolism; pathology; Cadherins; metabolism; Chromones; pharmacology; Doxorubicin; pharmacology; Epithelial-Mesenchymal Transition; drug effects; Humans; MCF-7 Cells; Morpholines; pharmacology; Phosphatidylinositol 3-Kinases; antagonists & inhibitors; Proto-Oncogene Proteins c-akt; metabolism; Signal Transduction; drug effects; Snail Family Transcription Factors; Transcription Factors; metabolism
- From: Acta Academiae Medicinae Sinicae 2012;34(4):319-323
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effect of LY294002 on the adriamycin- induced epithelial-mesenchymal transition in human breast carcinoma cells.
METHODSHuman breast carcinoma cells MCF-7 was cultured in vitro and then exposed to adriamycin with or without LY294002. The protein expression levels of Akt, phosphorylated-Akt (p-Akt), Snail, and E-cadherin was detected by Western blot analysis. The mRNA expressions of Snail and E-cadherin were determined by RT-PCR.
RESULTSAdriamycin significantly increased the protein expression of Snail and depressed the protein expression of E-cadherin (P<0.05). The pre-treatment with LY294002 significantly reversed the changes of activities and levels of the above proteins (P<0.05).
CONCLUSIONLY294002 could reverse the adriamycin-induced epithelial-mesenchymal transition in human breast carcinoma cells by regulating the expressions of Snail and E-cadherin through suppressing PI3K/Akt signaling pathway.
