Effect of SiO2 on expression of MMP-9/TIMP-1 in human alveolar macrophages in vitro.
- Author:
Li ZHANG
1
;
Xiao-Hui HAO
;
Xian-Hua WANG
;
Fang YANG
;
Zhi-Yi GUO
;
Xin PEI
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Humans; Macrophages, Alveolar; drug effects; metabolism; Matrix Metalloproteinase 9; metabolism; Silicon Dioxide; toxicity; Silicosis; pathology; Tissue Inhibitor of Metalloproteinase-1; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(6):341-344
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in human silicotic alveolar macrophages (AM).
METHODSHuman alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3 h, 6 h, 12 h, 18 h, 24 h and 36 h. The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.
RESULTSThe expressions of MMP-9 in the AM increased clearly along with the time, reached peak at 24 h when detected with zymography (average optical density: 3.061+/-0.153 vs 2.851+/-0.164, P<0.05); and reached peak at 18h when detected with immunological method (average optical density: 0.386+/-0.037 vs 0.322+/-0.034, P<0.05). The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P>0.05).
CONCLUSIONSiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM.
