Effects of occupational microwave irradiation on heat shock protein 70 expressions in rat hippocampus.
- Author:
Yu-tong HAO
1
;
Li-ping PEI
;
Chun-hai CHEN
;
Xue-sen YANG
;
Guang-bin ZHANG
;
Zhao-hui DENG
;
Zheng-ping YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; HSP70 Heat-Shock Proteins; genetics; metabolism; Hippocampus; metabolism; radiation effects; Microwaves; adverse effects; RNA, Messenger; genetics; Rats; Rats, Wistar
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(9):553-556
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the change of heat shock protein (HSP)70 expression after exposure to occupational microwave in rats hippocampus, and explore the role of HSP70 in the mechanism of bio-effect of microwave irradiation.
METHODSThe animal model was established by whole body exposures in 90, 5 W/cm(2) microwave irradiation field for 20 min in rats. Changes of the mRNA of hsp70 expressions in rat hippocampus at different time were studied by RT-PCR, and the protein change by Western blot.
RESULTSThe mRNA and protein expression of hsp70 in rat hippocampus increased after 90 W/cm(2) and 5 W/cm(2) microwave irradiation for 20 min. The anal temperature and the value of SAR increased significantly. These changes were positively correlated with power and irradiation time of microwave. The results indicated that microwave irradiation led to HSP70 syntheses effectively.
CONCLUSIONMicrowave irradiation can obviously induce the thermal effect and activate HSP70, and initiate the endogenous protective mechanism of central nervous system.
