Black rice anthocyanidins prevent retinal photochemical damage via involvement of the AP-1/NF-kappaB/Caspase-1 pathway in Sprague-Dawley Rats.
10.4142/jvs.2013.14.3.345
- Author:
Hao JIA
1
;
Wei CHEN
;
Xiaoping YU
;
Xiuhua WU
;
Shuai LI
;
Hong LIU
;
Jiru LIAO
;
Weihua LIU
;
Mantian MI
;
Longjian LIU
;
Daomei CHENG
Author Information
1. Department of Public Health, Chengdu Medical College, Chengdu City, 610050, China. cyggwsyxp@sina.com
- Publication Type:Original Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
- Keywords:
apoptosis;
black rice anthocyanidins;
Caspase-1;
photochemical damage;
retina
- MeSH:
Animal Feed/analysis;
Animals;
Anthocyanins/administration & dosage/*pharmacology;
Antioxidants/administration & dosage/*physiology;
Blotting, Western;
Caspase 1/*genetics/metabolism;
Diet;
Dietary Supplements/analysis;
I-kappa B Proteins/genetics/metabolism;
NF-kappa B/*genetics/metabolism;
Neoplasm Proteins/genetics/metabolism;
Nucleocytoplasmic Transport Proteins/genetics/metabolism;
Oryza sativa/chemistry;
Proto-Oncogene Proteins c-fos/genetics/metabolism;
Proto-Oncogene Proteins c-jun/genetics/metabolism;
Rats;
Rats, Sprague-Dawley;
Real-Time Polymerase Chain Reaction;
Retinal Diseases/etiology/*prevention & control;
Signal Transduction/*drug effects/radiation effects;
Transcription Factor AP-1/*genetics/metabolism
- From:Journal of Veterinary Science
2013;14(3):345-353
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of black rice anthocyanidins (BRACs) on retinal damage induced by photochemical stress are not well known. In the present study, Sprague-Dawley rats were fed AIN-93M for 1 week, after which 80 rats were randomly divided into two groups and treated with (n = 40) or without BRACs (n = 40) for 15 days, respectively. After treatment, both groups were exposed to fluorescent light (3,000 +/- 200 lux; 25degrees C), and the protective effect of dietary BRACs were evaluated afterwards. Our results showed that dietary BRACs effectively prevented retinal photochemical damage and inhibited the retinal cells apoptosis induced by fluorescent light (p < 0.05). Moreover, dietary BRACs inhibited expression of AP-1 (c-fos/c-jun subunits), up-regulated NF-kappaB (p65) expression and phosphorylation of IkappaB-alpha, and decreased Caspase-1 expression (p < 0.05). These results suggest that BRACs improve retinal damage produced by photochemical stress in rats via AP-1/NF-kappaB/Caspase-1 apoptotic mechanisms.
