ClC-3 expression in the cell cycle of nasopharyngeal carcinoma cells.
- Author:
Li-Wei WANG
1
;
Li-Xin CHEN
;
Tim JACOB
Author Information
1. Department of Physiology and Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China. WANGLW@cardiff.ac.uk
- Publication Type:Journal Article
- MeSH:
Adenosine Triphosphate;
pharmacology;
Cell Cycle;
physiology;
Chloride Channels;
metabolism;
physiology;
Chlorides;
metabolism;
Gene Expression Regulation, Neoplastic;
Humans;
Muscle Proteins;
metabolism;
physiology;
Nasopharyngeal Neoplasms;
genetics;
metabolism;
pathology;
Tumor Cells, Cultured
- From:
Acta Physiologica Sinica
2004;56(2):230-236
- CountryChina
- Language:English
-
Abstract:
The immunofluorescence approach, the confocal microscopy and the patch-clamp technique were used to investigate the expression of ClC-3 (one of the candidates of volume-activated chloride channels) and its relationships with the volume-activated chloride current and the capacity of regulatory volume decrease (RVD) in the cell cycle of nasopharyngeal carcinoma cells (CNE-2Z cells). The results indicated that CNE-2Z cells expressed ClC-3. ClC-3 was located predominantly inside the cells but not in the membrane. Both the expression level and the distribution of ClC-3 were cell cycle dependent. ClC-3 expression was low in G1 but high in S phase. The cells in G2/M phase possessed an intermediate level of the expression. ClC-3 expression level was negatively correlated to the RVD capacity and amplitude of the volume-activated chloride current in the cell cycle. The results suggest that ClC-3 may be an important factor in the regulation of cell cycle progression, but that it is probably not the chloride channel associated with RVD in these cancer cells.
