Effect of repeated acute hypoxic treatment on the expression of alpha-synuclein in the mouse brain cortex.
- Author:
Shun YU
1
;
Xue-Mei LIU
;
Yao-Hua LI
;
Guo-Wei LU
;
Biao CHEN
Author Information
1. Department of Neurobiology, Beijing Institute of Geriatrics, Xuan Wu Hospital, Capital University of Medical Sciences.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain;
blood supply;
Brain Ischemia;
metabolism;
Cerebral Cortex;
metabolism;
Ischemic Preconditioning;
Mice;
Mice, Inbred BALB C;
Nerve Tissue Proteins;
biosynthesis;
genetics;
Neurons;
metabolism;
Phosphoproteins;
biosynthesis;
genetics;
Random Allocation;
Synucleins;
alpha-Synuclein
- From:
Acta Physiologica Sinica
2004;56(2):263-268
- CountryChina
- Language:Chinese
-
Abstract:
An anti-alpha-synuclein (alpha-SYN) monoclonal antibody produced in our laboratory was used to investigate the effect of repeated acute hypoxic treatments on the expression of alpha-SYN in the mouse cerebral cortex. Western blot analysis showed that the expression levels of alpha-SYN in the cortex changed accordingly upon hypoxic exposure times, as that the alpha-synuclein level significantly increased after the first hypoxic exposure and then dropped down to the background level after the fourth hypoxic exposure. Immunohistochemical staining revealed that the alpha-SYN-immunopositive substance was localized not only in the nerve endings, but also within the nuclei of some neurons. The cell density of the neurons with alpha-SYN immunopositive nuclei was increased significantly after the first hypoxic exposure but returned back to control levels after the fourth hypoxic exposure. Our results indicate that both of the alpha-SYN expression level in the brain and the number of the neurons with alpha-SYN positive nuclei are affected by the repeated acute hypoxic treatments and that this modification is hypoxic time-dependent. The mechanism and the physiological significance underlying these changes need to be further investigated.
