Volume-activated Cl- current in migrated nasopharyngeal carcinoma cells.
- Author:
Jian-Wen MAO
1
;
Li-Wei WANG
;
Xue-Rong SUN
;
Lin-Yan ZHU
;
Pan LI
;
Ping ZHONG
;
Si-Huai NIE
;
Tim JACOB
;
Li-Xin CHEN
Author Information
1. Laboratory of Cell Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma;
drug therapy;
metabolism;
pathology;
Cell Cycle;
drug effects;
physiology;
Cell Division;
drug effects;
Cell Movement;
drug effects;
Cell Size;
drug effects;
Chloride Channels;
antagonists & inhibitors;
metabolism;
physiology;
Chlorides;
metabolism;
Humans;
Nasopharyngeal Neoplasms;
drug therapy;
metabolism;
pathology;
Nitrobenzoates;
pharmacology;
Patch-Clamp Techniques;
Tamoxifen;
pharmacology;
Tumor Cells, Cultured
- From:
Acta Physiologica Sinica
2004;56(4):525-530
- CountryChina
- Language:English
-
Abstract:
The transwell chamber migration assay and the patch-clamp technique were used to investigate the volume-activated Cl(-) current (I(Cl.vol)) in migrated nasopharyngeal carcinoma cells (CNE-2Z). 47% hypotonic solution activated a ICl.vol in the migrated CNE-2Z cells. Compared with the control cells (non-migrated), the properties of this current and the sensitivity to Cl(-) channel blockers were changed. The current density in migrated CNE-2Z cells was higher than that in non-migrated cells. The current was almost completely inhibited by extracellular application of adenosine-5'-triphosphate (ATP, 10 mmol/L), 5-nitro-2-3-phenylpropylamino benzoic acid (NPPB, 100 mmol/L) and tamoxifen (30 mmol/L) in all voltage steps applied. The inhibition of NPPB and tamoxifen on the current was stronger in migrated cells than that in non-migrated cells. The permeability sequence of the four anions was Br(-)>Cl(-)> I (-)>Gluconate. The sequence was different from that of the non-migrated cells (I(-)> Br(-)> Cl(-)> Gluconate). The results suggest that volume-activated chloride channels may be involved in the CNE-2Z cell migration.
