Analysis of pulsed-field gel electrophoresis molecular subtyping of Shigella strains in Shenzhen.
- Author:
Quan-xue LAN
1
;
Qing-hua HU
;
Xiao-lu SHI
;
Bing WANG
;
Yi-man LIN
;
Jin-quan CHENG
;
Shun-xiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Typing Techniques; China; Electrophoresis, Gel, Pulsed-Field; methods; Feces; microbiology; Humans; Shigella; classification; isolation & purification
- From: Chinese Journal of Preventive Medicine 2008;42(5):317-320
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the genetic relations of Shigella isolated from Shenzhen in 2001-2006 and develop primary molecular subtyping surveillance network of Shigella.
METHODSChromosomal DNAs from 55 isolated in agarose were digested with the restriction enzyme Xba I, and then were analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns were clustered using BioNumerics software.
RESULTSAll 41 distinctive PFGE patterns were identified among 55 strains. 32 strains belonged to one cluster. Differences were observed in other strains.
CONCLUSIONBoth genetic-related clones and non-related clones of Shigella existed in Shenzhen. The development of PFGE molecular subtyping surveillance network would contribute to the active surveillance, outbreak investigation and source tracking for Shigellosis.
