Association between polycyclic aromatic hydrocarbons exposure levels and nucleoplasmic bridge and nuclear bud frequencies in coke-oven workers.
- Author:
Hua-wei DUAN
1
;
Shu-guang LENG
;
Zu-fei PAN
;
Yong NIU
;
Ping BIN
;
Yu-fei DAI
;
Yu-xin ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alcohol Drinking; Cell Nucleus; Coke; DNA Damage; Female; Humans; Lymphocytes; Male; Micronucleus Tests; Occupational Exposure; analysis; Polycyclic Aromatic Hydrocarbons; poisoning; Smoking
- From: Chinese Journal of Preventive Medicine 2008;42(6):391-394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo seek new effect biomarkers as to evaluating the chromosomal damage in peripheral blood lymphocytes in coke-oven workers who were exposed to polycyclic aromatic hydrocarbons (PAHs).
METHODSOne hundred and fifty-eight coke-oven workers and 69 controls were recruited in this study. Nucleoplasmic bridges and nuclear buds were counted as indicators of chromosomal damage in terms of cytokinesis-block micronucleus (CBMN) test. Occupational history, age, sex, smoking and alcohol using status of all subjects were collected by questionnaire.
RESULTSFrequencies of nucleoplasmic bridge in coke-oven workers were (9.41 +/- 3.73)% per hundred, and the frequencies of nuclear buds were (7.13 +/- 4.01)% per hundred, which were significantly higher (P < 0.01) than those of controls (1.88 +/- 1.49)% per hundred and (2.20 +/- 1.73)% per hundred respectively. The dose-effect relationships between nucleoplasmic bridges or nuclear buds and PAHs exposure levels were identified. Compared with male coke-oven workers, female workers had less nucleoplasmic bridges or nuclear buds. No effects of age, smoking and alcohol using were found on nucleoplasmic bridges or nuclear buds among coke-oven workers.
CONCLUSIONNucleoplasmic bridges and nuclear buds might be effect biomarkers in coke-oven workers.