Development of bovine blood lead component analysis standard reference materials.
- Author:
Jian-ping LI
1
;
Yi-qun WU
;
Yong-gang ZHAO
;
Qi-Hong
;
Ai-jun CHI
Author Information
- Publication Type:Journal Article
- MeSH: Animal Feed; Animals; Blood Chemical Analysis; standards; Cattle; Lead; blood; Male; Models, Animal; Reference Standards
- From: Chinese Journal of Preventive Medicine 2008;42(6):410-413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop the first grade standard analytical reference material of bovine blood lead.
METHODSAccording to standards and technology specification of primary standard reference material (JJG1006-1994), ISO 17511, and volume-effect relationship, a bovine blood lead model was developed by adding acetate lead in the feed in dose of 2-5 mg x kg(-1) x d(-1). Cow blood was collected when blood lead concentration went up to low, medium and high concentration range (90-100, 190-200, 280-300 microg/L). Blood sample was kept in tube and frozen after irradiation. The samples were tested for homogeneity and stability. ID-MS method was used to measure the lead concentration through comparison with two standard lead samples from the USA NIST SRM 955b.
RESULTSSamples of the three lead concentrations showed uniformity by single factor analysis of variance (F = 0.61, 1.64, 0.28, respectively, P > 0.05) . After 14 months monitoring, the RSD was 0.85%, 1.05% and 0.49% (t = 0.787, 1.132, 0.854, respectively, P > 0.05). The characteristic and indefinite values were 102.4 +/- 5.5; 181.2 +/- 4.0; 304.5 +/- 3.9, respectively (unit: microg/L). The reproduction of the two USA NIST SRM 955b samples further showed our methods and procedures were correct. Our sample was stabile for four days at room temperature.
CONCLUSIONThe standard reference material of bovine in our research had reached the national standard material requirements.
