Molecular subtyping of Staphylococcus aureus isolated from a severe food-poisoning.
- Author:
Ying ZHANG
1
;
Zi-Yao MO
;
Xing-Lin PANG
;
Zhi-Ai DENG
;
Xin-Qiang ZHANG
;
Shou-Yi CHEN
;
Ming WANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Typing Techniques; China; Electrophoresis, Gel, Pulsed-Field; Enterotoxins; Humans; Staphylococcal Food Poisoning; epidemiology; microbiology; Staphylococcus aureus; classification; genetics; isolation & purification
- From: Chinese Journal of Preventive Medicine 2008;42(9):672-676
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the molecular types of Staphylococcus aureus isolated from a severe food-poisoning and to trace the possible strains.
METHODSReal-time PCR was applied to detect nuc gene as a specific marker for S. aureus, mecA gene encoding methicillin resistance and 5 other genes encoding staphylococcal enterotoxins (sea, seb, see, sed, see). Isolates were also performed with 16S rRNA oligonucleotide sequence analyzing by DNAStar MegAlign 5.0 software and pulse-field gel electrophoresis (PFGE) by BioNumerics Version 4.0 software.
RESULTSThe nuc gene was detected from the 10 isolated strains, sea and seb genes were detected from 7 strains. There were 4 16 S rRNA types and 5 PFGE types found from all the strains.
CONCLUSIONSThree relative S. aureus strains were involved in the severe food-poisoning at least. Molecular subtyping might give a molecular epidemiological evidence and support the source tracing of an outbreak.
