Expressions of adiponectin and its receptors in the retina of normal and type 1 diabetic mice.
- Author:
Tao LIN
1
;
Feng HE
;
Bo LEI
Author Information
- Publication Type:Journal Article
- MeSH: Adiponectin; metabolism; Animals; Diabetes Mellitus, Experimental; metabolism; Diabetes Mellitus, Type 1; metabolism; Diabetic Retinopathy; metabolism; pathology; Male; Mice; Mice, Inbred C57BL; Receptors, Adiponectin; metabolism; Retina; metabolism
- From: Journal of Southern Medical University 2012;32(11):1543-1547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of adiponectin and its receptors (AdipoRs) in the retina of normal mice and mice with type 1 diabetes mellitus (T1DM).
METHODSC57BL/6 mice were randomly divided into control group and streptozotocin-induced T1DM group. Two months after the modeling, the total protein and adiponectin protein expression in the retina and choroid were measured using BCA method and enzyme-linked immunosorbent assay, respectively. Quantitative RT-PCR was performed to detect the mRNA expressions of AdipoRs in the retina and choroid, and Western blotting was employed to examine the protein expressions of AdipoRs in the retina.
RESULTSAdiponectin and AdipoRs proteins were expressed in the retina and choroid in normal mice. The expressions of adiponectin and AdipoR1 were up-regulated in the retina of mice with T1DM while AdipoR2 expression exhibited no significant changes.
CONCLUSIONAdiponectin and AdipoR1 may play an important role in the evolvement of type 1 diabetic retinopathy.
