Comparative study of retinopathy in C57BL/6 and eNOS-knockout mouse models of type 1 diabetes mellitus.
- Author:
Yu LIU
1
;
Tao LIN
;
Bo LEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diabetes Mellitus, Experimental; complications; pathology; Diabetes Mellitus, Type 1; complications; pathology; Diabetic Retinopathy; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Nitric Oxide Synthase Type III; genetics; Retinal Ganglion Cells; pathology
- From: Journal of Southern Medical University 2012;32(12):1683-1688
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the retinal function and retinal vascular pathologies in C57BL/6 and eNOS-knockout (eNOS(-/-)) mouse models of type 1 diabetes mellitus (T1DM) induced by streptozotocin (STZ).
METHODST1DM models were established in 6- to 8-week-old C57BL/6 and eNOS(-/-) mice by intraperitoneal STZ injection. Electroretinogram (ERG) examination, fluorescein angiography (FFA), immunofluorescence staining and retinal ganglion cell counts were carried out before and after STZ injection.
RESULTSDiabetic C57BL/6 and eNOS(-/-) mice showed significantly lowered a-wave and b-wave amplitude in ERG and reduced number of retinal ganglion cells (P<0.05), and the retinal vessels in diabetic eNOS(-/-) mice became tortuous. Compared with diabetic C57BL/6 mice, diabetic eNOS(-/-) mice showed more severe pathological changes in retinal function and retinal vessels with also more rapid onset of pathologies.
CONCLUSIONCompared with C57BL/6 mouse models, eNOS(-/-) mouse models of T1DM can better represent the occurrence and development of diabetic retinopathy, thus providing an ideal model for diabetes and diabetic retinopathy studies.
