Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells.
- Author:
Xili WU
1
;
Wansen SUN
;
Bingyu YE
;
Peng AN
;
Xingmin SHI
;
Rongguo FU
;
Zhu WANG
;
Chenglin QIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Cyclin D1; metabolism; Cyclin-Dependent Kinase 2; metabolism; Cyclin-Dependent Kinase Inhibitor p21; metabolism; Cyclin-Dependent Kinase Inhibitor p27; metabolism; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation; drug effects; Mesangial Cells; cytology; drug effects; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2012;32(12):1699-1703
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Qufengtongluo Recipe (QFTLR) on the expressions of cell cycle regulatory proteins in rat mesangial cells (MCs) in vitro and investigate the mechanism by which QFTLR inhibits MC proliferation.
METHODSUsing the methods of serum pharmacology, we studied the expressions of cell cycle regulatory proteins in rat MCs treated with QFTLR by laser scanning confocal microscope and immunohistochemistry.
RESULTSCompared with the normal control cells, the cells challenged with lipopolysaccharide (LPS) showed significantly enhanced expressions of cyclin D1, CDK2, and P21 (P<0.01) and obviously lowered protein expression of P27 (P<0.01). Treatment of the LPS-challenged cells with QFTLR and benazepril both resulted in significantly attenuated expressions of cyclin D1, CDK2, and P21 and obvious increase of P27 expression (P<0.05 or P<0.01), but QFTLR produced stronger effects than benazepril in regulating of cyclinD1, P21 and P27 protein expressions (P<0.05 or P<0.01).
CONCLUSIONQFTLR inhibits rat MC proliferation in vitro possibly by down-regulating the cellular expressions of cyclin D1, CDK2, and P21 and up-regulating the expression of P27 protein.
