Effect of atorvastatin on eNOS synthesis in organs of aging rats with myocardial ischemia-reperfusion.
- Author:
Jinyao ZHANG
1
;
Hao WANG
;
Ping YE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Atorvastatin Calcium; Female; Heptanoic Acids; administration & dosage; pharmacology; Kidney; metabolism; Liver; metabolism; Male; Myocardial Reperfusion Injury; drug therapy; metabolism; Myocardium; metabolism; Nitric Oxide Synthase Type III; genetics; metabolism; Pyrroles; administration & dosage; pharmacology; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2012;32(12):1708-1712
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of atorvastatin on eNOS synthesis in the vital organs of aging rats and explore its mechanism for protection against myocardial ischemia-reperfusion injury.
METHODSTwenty-month-old Wistar rats were given daily atorvastatin lavage for 4 months. Myocardial ischemia-reperfusion model was established by ligating the coronary artery. The rats were randomized into normal control group, untreated model group, medication without surgery group, and atorvastatin-treated surgical group. The content of eNOS in the heart, liver and kidneys was detected by Western blotting, and eNOS mRNA expression by RT-PCR. The effects of different doses of atorvastatin on eNOS expressions were also evaluated.
RESULTSAtorvastatin significantly promoted eNOS synthesis in the heart, liver and kidney of the rats (P<0.05) regardless of myocardial ischemia-reperfusion. A higher dose of atorvastatin caused a more obvious increase of eNOS protein and mRNA expression in the vital organs of the aging rats (P<0.05).
CONCLUSIONAtorvastatin can increase eNOS synthesis in the vital organs of aging rats, which partially explains the organ-protective effect of atorvastatin against myocardial ischemia- reperfusion.
