Apoptosis of Cultured Rabbit Keratocyte Induced by Mitomycin-C.
- Author:
Mee Kum KIM
1
;
Won Ryang WEE
;
Min Suk KIM
;
In Suk PARK
;
Jin Hak LEE
Author Information
1. Department of Ophthalmology, College of Medicine, Seoul National University.
- Publication Type:Original Article
- MeSH:
Apoptosis*;
DNA;
Electrophoresis;
Mitomycin*;
Propidium;
Wound Healing
- From:Journal of the Korean Ophthalmological Society
2000;41(2):332-338
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To determine whether mitomycin-C[MMC]treatment induces apoptosis in cultured keratocytes. Cultured rabbit keratocytes were exposed to MMC[up to 0.4 milligram/milliliter]or phosphate-buffered saline[PBS]for 5 minutes. Light and transmission electron microscopic examination, DNA electrophoresis, and flow cytometric analysis with propidium iodide staining were performed 24 hours after MMC or PBS treatment. A characteristic findings consistent with apoptosis were observed under both light and electron microscopic examination and DNA ladder pattern was shown on electrophoresis. The average percentages of apoptosis measured by flow cytometric analysis were as follows;0.16 +/-0.08%in PBS, 0.23 +/-0.13%in 0.1 milligram/milliliter MMC, 0.50 +/-0.13%in 0.2 milligram/milliliter M M C , and 6.50 +/-1.57%in 0.4 milligram/milliliter MMC. Significant differences were shown in the percentage of apoptosis among the groups based on Kruskal-Wallis test[p=0.005]. Clinically relevant doses of MMC induces apoptosis in cultured keratocytes proportionally. This results suggest that MMC may modulate corneal wound healing process by accelerating the resolution phase of wound healing.
