High-mobility group box protein 1 in synergy with interleukin-1β promotes interleukin-8 expression in human airway epithelial cells in vitro.
- Author:
Dandan ZHANG
1
;
Haijin ZHAO
;
Liqin ZHOU
;
Jiafu SONG
;
Hangming DONG
;
Fei ZOU
;
Shaoxi CAI
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; drug effects; Cell Line; Epithelial Cells; drug effects; metabolism; HMGB1 Protein; pharmacology; Humans; Inflammation; Interleukin-1beta; pharmacology; Interleukin-8; metabolism; RNA, Messenger
- From: Journal of Southern Medical University 2012;32(12):1764-1767
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo test the effect of high-mobility group box protein 1 (HMGB1) alone or in synergy with interleukin-1β (IL-1β) on the expression of IL-8 in human airway epithelial cells in vitro.
METHODSHuman airway epithelial 16HBE and A549 cell lines were incubated with HMGB1 (100 ng/ml) in the absence or presence of IL-1β (10 ng/ml) for 24 h, and the changes of IL-8 mRNA and protein expressions were assessed using quantitative PCR and enzyme-linked immunosorbent assay (ELISA).
RESULTSIn the two human airway epithelial cell lines, HMGB1 alone did not produce obvious effect on the expression of IL-8, but in the presence of IL-1β, HMGB1 caused a significant increase of IL-8 expressions at both the mRNA and protein levels.
CONCLUSIONHMGB1 in synergy with IL-1β increases the expression of IL-8 in human airway epithelial cells, which provides new evidence that HMGB1 contributes to neutrophilic airway inflammation by regulating IL-8 expression.
