Anti-Mur screening and Mur antigen genotyping of voluntary blood donors in Guangzhou.
- Author:
Ling WEI
1
;
Yanli JI
;
Chunyan MO
;
Runqing ZHANG
;
Yang ZHAO
;
Hong LUO
;
Zhen WANG
;
Guangping LUO
Author Information
- Publication Type:Journal Article
- MeSH: Blood Donors; Blood Group Antigens; genetics; immunology; China; Genotype; Genotyping Techniques; Humans; Multiplex Polymerase Chain Reaction; Phenotype
- From: Journal of Southern Medical University 2012;32(12):1833-1835
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the frequency of anti-Mur and Mur antigen among blood donors in Guangzhou to provide evidence for guiding clinical transfusion and prenatal screening.
METHODSDG Gel Coombs cards were used to screen active anti-Mur at 37 degrees celsius; from 2725 blood donors. Multiplex ligation-dependent probe amplification (MLPA) was used to genotype Mur antigen from 91 blood donors, and human anti-Mur serum was used to verify the phenotypes deduced from the genotypes.
RESULTSThe frequency of anti-Mur and genotyped Mur antigen was 0.04% (1/2725) and 6.59% (6/91), respectively, and the phenotyping results were consistent with the genotyping results.
CONCLUSIONThe blood donors in Guangzhou show a low frequency of anti-Mur and a relatively high frequency of Mur antigen. Genotyping using MLPA allows Mur antigen genotyping when commercial anti-Mur is not available.