Interferon-γ enhances human γδ T cell-mediated osteosarcoma cell killing in vitro.
- Author:
Zhaoxu LI
1
;
Jicun TANG
;
Zhaoming YE
Author Information
- Publication Type:Journal Article
- MeSH: Bone Neoplasms; metabolism; Cell Line, Tumor; Cytotoxicity, Immunologic; Humans; Interferon-gamma; pharmacology; Osteosarcoma; immunology; metabolism; Receptors, Antigen, T-Cell, gamma-delta; immunology; T-Lymphocytes, Cytotoxic; cytology; drug effects; immunology; fas Receptor; metabolism
- From: Journal of Southern Medical University 2013;33(1):22-25
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the cytotoxic effect of γδ T cells from osteosarcoma patients against interferon-γ (IFN-γ)-treated osteosarcoma cells in vitro.
METHODSHuman γδ T cells were amplified by zoledronate from peripheral blood cells of osteosarcoma patients. The expression of Fas on the osteosarcoma cells were measured by flow cytometry and quantitative real-time PCR analysis before and after IFN-γ treatment. The cytotoxicity of γδ T cells against osteosarcoma cells was evaluated with LDH assay.
RESULTSIFN-γ significantly enhanced the susceptibility of the osteosarcoma cell lines HOS and U2OS to the cytotoxicity of γDelta; T cells from osteosarcoma patients (P<0.01). IFN-γ obviously up-regulated the expression of Fas in HOS and U2OS cells (P<0.01). Anti-FasL mAb failed to inhibit the cytotoxicity of γδ T cells in untreated osteosarcoma targets (P>0.05), but significantly impaired γδ T cell cytotoxicity in IFN-γ pre-treated osteosarcoma targets (P<0.01).
CONCLUSIONIFN-γ can enhance the cytotoxic effect of human γδ T cells from osteosarcoma patients against osteosarcoma cells in vitro.
