Effect of Biejiajian Pills on Wnt/β-catenin signal pathway and DKK-1 and FrpHe gene expressions in hepatocellular carcinoma cells.
- Author:
Songqi HE
1
;
Yang CHENG
;
Yun ZHU
;
Qin FAN
;
Haitao SUN
;
Wenyan JIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Hepatocellular; genetics; metabolism; pathology; Drugs, Chinese Herbal; pharmacology; Hep G2 Cells; Humans; Intercellular Signaling Peptides and Proteins; metabolism; Liver Neoplasms; genetics; metabolism; pathology; Male; Proto-Oncogene Proteins; metabolism; Rats; Rats, Wistar; Wnt Proteins; metabolism; Wnt Signaling Pathway; drug effects; beta Catenin; metabolism
- From: Journal of Southern Medical University 2013;33(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Biejiajian Pills on Wnt signal pathway and its inhibitory gene (DKK-1 and FrpHe) expressions and explore the mechanism underlying the action of Biejiajian Pills to suppress the invasiveness of hepatocellular carcinoma.
METHODSTwenty-four Wistar rats were randomized equally into 3 groups for gavage of normal saline and Biejiajian Pills at 20- and 10-fold clinical doses for 3 days. Blood samples were then collected from the rats, and the serum was separated and added in HepG2 cell cultures. After 48 h of culture, the cells were collected to determine the cellular content of β-catenin protein using flow cytometry and detect DKK-1 and FrpHe mRNA expressions using qRT-PCR.
RESULTSHepG2 cells cultured in the presence of sera from rats fed with Biejiajian Pills showed significantly lowered β-catenin protein expression and obvious down-regulation of DKK-1 mRNA expression, and the effect was correlated with the doses of the drug administered. The expression of FrpHe mRNA showed no significant differences between the 3 groups.
CONCLUSIONSBiejiajian Pills can effectively inhibit the invasiveness and migration of hepatocellular carcinoma cells, which is closely related to decreased expressions of β-catenin and DKK-1 to cause block of the Wnt/β-catenin signal pathway.
