The changes in the prostaglandin level and the prostaglandin transfer factor mRNA expression in the intestinal mucosa in scalded rats.

De-sheng Meng; Shi-liang Wang

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The changes in the prostaglandin level and the prostaglandin transfer factor mRNA expression in the intestinal mucosa in scalded rats.

Author: De-Sheng MENG ¹ ; Shi-Liang WANG
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1. Institute of Burn Research, Southwestern Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China.
Publication Type:Journal Article
MeSH: Animals; Burns; metabolism; pathology; Intestinal Mucosa; metabolism; pathology; Organic Anion Transporters; metabolism; Prostaglandins; analysis; RNA, Messenger; metabolism; Rats; Rats, Wistar
From: Chinese Journal of Burns 2003;19(5):279-281
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the changes in the PGE(2) and PGI(2), TXA(2) levels and PGT mRNA expression in the intestinal mucosa in scalded rats.
METHODSWistar rats inflicted with TBSA 30% III degree scald were employed as the model. The PGE(2) and PGI(2) and TXA(2) contents in the intestinal mucosa were measured by radioimmunoassay, and the expression of PGT mRNA was detected by in situ hybridization.
RESULTSThe PGE(2) and PGI(2) levels in intestinal mucosa were increased at 12 postburn hours (PBHs) and thereafter decreased dramatically (P < 0.05). The TXA(2) level in intestinal mucosa of scalded rats was obviously higher than that of normal level at 24 and 48 PBHs (P < 0.05), and the expression of PGT mRNA seemed to be increased after scalding.
CONCLUSIONThe decrease of PGE(2) level and the increase of TXA(2) level in the intestinal mucosa of scalded rats might be involved in rat mucosal injury, and PGT played an important role in the regulation of PGs levels.