Study on the injurious effect of a self designed micro-skin machine on the epithelia.
- Author:
Jian-she CHEN
1
;
Jin-song CHEN
;
Xian-zhi LIU
;
Zong-ren ZHANG
;
Guang-yu SHEN
;
Hong-jie DUAN
;
Yong-yue SU
;
Yue-ming LIU
;
Gen-fa LV
Author Information
- Publication Type:Journal Article
- MeSH: Burns; surgery; Cell Division; Epithelium; pathology; Humans; Microscopy, Electron; Skin; ultrastructure; Skin Transplantation; methods; Wound Healing
- From: Chinese Journal of Burns 2003;19(6):355-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the injury on micro-skin induced by a self designed micro-skin machine.
METHODSMicro-skin was produced either with the machine or by hand. Cells at the edge of micro-skin were observed by transmission electron microscope. succinic dehydrogenase activity in supernatant of cultivated cells was analyzed, and the cell proliferation of micro-skin was assessed by (3)H-TdR. Twenty patients were enrolled in the study for the observation of the wound healing time between the two groups of micro-skin after being grafted.
RESULTSTransmission electron microscope examination revealed that the cellular injury at the edge of the micro-skin in machine-made group was mild compared with that in man-made group. (3)H-TdR rate was elevated but the activity of succinic dehydrogenase in the supernatant of cultured cells decreased in supernatant of cultured cells of machine produced micro-skin. Wound healing time was shortened in machine made group. (P < 0.05).
CONCLUSIONThe cellular injury at the edge of micro-skin in the machine made group was mild when compared with that in the man-made group with cell proliferation accelerated and wound healing time shortened.
