Study of biosensor technology on the detection of endotoxin-neutralizing materials.
- Author:
Gen-fa LU
1
;
Xiao-yun GONG
;
Guo WEI
;
Ning WANG
;
Guang-xia XIAO
;
Jiang ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Proteins; analysis; Biosensing Techniques; methods; Endotoxins; analysis; Lipid A; analysis; Polymyxin B; analysis; Reproducibility of Results; Sensitivity and Specificity
- From: Chinese Journal of Burns 2004;20(1):23-25
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the application of biosensor technology in the determination of endotoxin-neutralizing materials.
METHODSAfter mixing polymyxin B (PMB) with endotoxin in certain concentration, the neutralizing ratio of PMB to endotoxin was assessed by biosensor technique and limulus amebocyte lysate test respectively. The results from the two methods were compared.
RESULTSThe neutralizing ratio of PMB to endotoxin as assessed by biosensor technology was 0.35 microg to 1 ng, while that by dynamic turbidimetric and chromogenic limulus amebocyte lysate (LAL) technique was 0.5 mg to 1 ng and 1 mg to 1 ng, respectively. The results obtained by biotechnology were similar to that by biosensor technique.
CONCLUSIONBiosensor technology was an accurate, convenient and rapid method for the determination of potency of endotoxin-neutralizing materials.
