Effect of lipopolysacharide on the biological features and growth factor secretion power of U937 cell line.
- Author:
Jia MING
1
;
Xu-Sheng LIU
;
Liang LIU
;
Hui XU
;
Xin-Ze RAN
;
Tian-Min CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Division; drug effects; Humans; Lipopolysaccharides; pharmacology; Transforming Growth Factor beta; analysis; secretion; Transforming Growth Factor beta1; U937 Cells; drug effects; secretion; Vascular Endothelial Growth Factor A; analysis; secretion
- From: Chinese Journal of Burns 2004;20(2):92-94
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of lipopolysacharide (LPS) in different concentrations on the biological features and growth factor secretion power of U937 cell line.
METHODSIn vitro cultured U937 cells were stimulated by 0 (as control), 0.1, 1.0, 10.0, 50.0 and 100 micro g/ml LPS respectively for 24 hours. Thereafter, the cell proliferation ability was determined by MTT method. The cell apoptosis rate was determined by flow cytometry. The changes in the contents of transforming growth factor beta(1) (TGFbeta(1)) and vascular endothelial growth factor (VEGF) of the supernatant of the cell culture were assessed by ELISA.
RESULTSApoptosis and TGFbeta(1) secretion could be induced by LPS in dose of 0.1 to 100 micro g/ml when compared with that without LPS challenge (P < 0.05 - 0.01). In detail, LPS in lower dose (0.1, 1.0 and 10.0 micro g/ml) could promote the proliferation of U937 (P < 0.05 - 0.01) but exerted no effect on VEGF secretion. In contrary, LPS in high dose (50 and 100 micro g/ml) could promote VEGF secretion (P < 0.01) but exerted no effects on the proliferation of U937 cells.
CONCLUSIONU937 cells could be activated to increase the secretion of TGFbeta(1) by LPS in optimal dose of 0.1 - 10.0 micro g/ml, but the secretion of VEGF could only be promoted by LPS in higher concentration.