Construction of Runx1 transgenic mice.
- Author:
Xiaoju TANG
1
;
Junli LI
;
Xiaojing LIU
;
Yi LEI
;
Qiaoli SU
;
Qingbo WU
;
Fengming LUO
Author Information
1. Laboratory of Cardiovascular Diseases, Research Center of Regeneration Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Core Binding Factor Alpha 2 Subunit;
genetics;
Female;
Genetic Vectors;
genetics;
Male;
Mice;
Mice, Inbred C57BL;
Mice, Transgenic;
genetics;
Microinjections;
Molecular Sequence Data;
Recombination, Genetic
- From:
Journal of Biomedical Engineering
2013;30(3):584-587
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to construct transgenic mouse model with target for Runxl gene. Runxl cDNA of mice was amplified by PCR from pcDNA3. 1 Flag Runx1 FL vector and inserted into ptetO7-Asc-IRES-EGFP vector to form a recombinant vector, and then the recombinant vector was injected into fertilized egg by microinjection technology to get a transgenic mouse. The results of PCR and Southern blot indicated that the Runx1 transgenic mouse was constructed successfully, and this could provide an important tool for studying the function of Runxl gene in vivo.
