Effect of hydroxyapatite nanoparticles on MII-stage porcine oocytes vitrification and the study of its mechanism.
- Author:
Weijie LI
1
;
Xinli ZHOU
;
Jiangjun DAI
;
Defu ZHANG
;
Baolin LIU
;
Haisong WANG
;
Li XU
Author Information
1. Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Survival;
physiology;
Cryopreservation;
methods;
veterinary;
Cryoprotective Agents;
pharmacology;
Durapatite;
pharmacology;
Female;
Fertilization in Vitro;
methods;
veterinary;
Metaphase;
Nanoparticles;
Oocytes;
cytology;
Swine;
Vitrification
- From:
Journal of Biomedical Engineering
2013;30(4):789-793
- CountryChina
- Language:Chinese
-
Abstract:
Nano-cryopreservation may become a new way in the next generation of cryopreservation technology. However, research using nanoparticles in oocytes vitrification has not been reported in the literature. In this study, HA nanoparticles with different diameters were added into cryoprotectant and M II-stage porcine oocytes were vitrified by Cryotop. The results showed that nanoparticles improved the survival rate of cryopreserved M II-stage porcine oocytes, but the difference between nanoparticles with different diameters of was not significant. In order to study the mechanism of nano-cryopreservation, the cooling rate of cryoprotectant was measured by ultra-fast temperature measurement system and the melting enthalpy of cryoprotectant was measured by differential scanning calorimeter (DSC). The results showed that the adding of nanoparitcles could not increase the cooling rate of cryoprotectant, but could decreases the amount of ice crystals during freezing and warming. Therefore, the mechanical injury within and outside cells might be effectively reduced.
