The effect of heme oxygenase-1 on BMSCs damaged by high-concentration glucose.
- Author:
Chunli HOU
1
;
Lei SHEN
;
Yangxiao WU
;
Mingcan YANG
;
Wen ZENG
;
Li LI
;
Wen CHEN
;
Chuhong ZHU
Author Information
1. Department of Anatomy, the Key Lab of Biomechanics, the Third Military Medical University, Chongqing 400038, China.
- Publication Type:Journal Article
- MeSH:
Cell Movement;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Culture Media, Conditioned;
pharmacology;
Glucose;
toxicity;
Heme Oxygenase-1;
metabolism;
Human Umbilical Vein Endothelial Cells;
cytology;
Humans;
Mesenchymal Stromal Cells;
cytology;
Up-Regulation;
Vascular Endothelial Growth Factor A;
metabolism
- From:
Journal of Biomedical Engineering
2013;30(4):798-802
- CountryChina
- Language:Chinese
-
Abstract:
This investigation was aimed to explore whether over-expression of 27heme oxygenase-1 (HO-1) could protect bone marrow mesenchymal stem cells(BMSCs)against injury induced by high-concentration glucose. We cultured BMSCs in high-concentration glucose medium, and up-regulated or inhibited HO-1 expression in BMSCs through its agonist or inhibitor. We detected the ability of BMSCs proliferation and secretion respectively by MTT and enzyme-linked immunosorbnent assay (ELISA). Then we detected the effect of BMSCs conditions medium on proliferation and migration of human umbilical vein endothelial cells (HUVECs) through scratch experiments and transwell assay. It was found that HO-1 over-expression could not only promote BMSCs proliferation, but also promote secretion of vascular endothelial growth factor (VEGF), and could further accelerate the proliferation and migration of HUVECs. It could be well concluded that HO-1-over-expressing BMSCs can not only inhibit damage induced by high-concentration glucose, but can promote the proliferation and migration of vascular endothelial cells through paracrine as well. The result indicated that HO-1-over-expressing BMSCs played an important role in the treatment of diabetic vascular complication.
