Study on immunogenicity elicited by a recombinant vaccine of rBCG-Rv3133c to fight against dormancy Mycobacterium tuberculosis.
- Author:
Yihao DENG
1
;
Hongyun HE
;
Bensi ZHANG
Author Information
1. Department of Human Anatomy, College of Preclinical Medicine, Dali University, Dali 671000, China. deng13032871868@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Bacterial;
blood;
Antigens, Bacterial;
genetics;
immunology;
BCG Vaccine;
immunology;
Bacterial Proteins;
genetics;
immunology;
Escherichia coli;
genetics;
metabolism;
Interferon-gamma;
immunology;
Mice;
Mice, Inbred BALB C;
Mycobacterium tuberculosis;
immunology;
Protein Kinases;
genetics;
immunology;
Recombinant Proteins;
immunology;
T-Lymphocyte Subsets;
immunology;
Tuberculosis;
prevention & control;
Vaccination;
Vaccines, Synthetic;
immunology
- From:
Journal of Biomedical Engineering
2013;30(4):817-821
- CountryChina
- Language:Chinese
-
Abstract:
To obtain a vaccine to defend from dormancy Mycobacterium tuberculosis, we constructed the recombinant Bacilli Calmette-Guérin (BCG) vaccine with Rv3133c encoding dormancy-correlated transcriptional regulatory protein DosR in Mycobacterium tuberculosis as a target gene, and evaluated its immunogenicity in BALB/c mice. In this study, we constructed the recombinant plasmids of rpMV361-Rv3133c using gene colon technology. We then transformed BCG strains with above-mentioned plasmids to obtain recombinant vaccine of rBCG-Rv3133c. We used the rBCG strains successfully constructed to vaccinate in BALB/c mice. 30d and 180d after immunization, the specific antibody titers were determined to investigate humoral responses induced by recombinant vaccine. We detected changes of splenocyte subsets of CD4+T, CD8+ T cells and cytokine of IFN-gamma secreted by splenocytes for evaluation of cellular immune responses. The results showed that the rBCG-Rv3133c was able to induce higher levels of antibody titer, stronger proliferative responses and higher IFN-gamma production comparing with BCG vaccine. The results also suggested that this recombinant vaccine was a more efficacious tuberculosis vaccine for further study.
