Preliminary establishment of an electrochemiluminescence immunoassay method for detection of influenza virus B.
- Author:
Ran CHEN
1
;
Jie WANG
1
;
Zhongming LIU
1
Author Information
1. Department of Medical Research, General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
- Publication Type:Journal Article
- MeSH:
Antibodies, Viral;
analysis;
Electrochemical Techniques;
Immunoassay;
methods;
Influenza B virus;
isolation & purification;
Luminescence
- From:
Journal of Biomedical Engineering
2013;30(5):968-971
- CountryChina
- Language:Chinese
-
Abstract:
To establish an electrochemiluminescence immunoassay (ECLIA) for the detection of influenza virus B (Flu B), we biotinylated the Flu B monoclonal antibody (mAb),labeled the paired Ab with ruthenium and used the streptavidin-coated microparticles in this study. The ECL intensity was linear with the concentrations of antigen of Flu B in the range of 0.55 microg/mL to 17.5 microg/mL, with a detection limit of 0.55 microg/mL (S/N= 3). The precision,sensitivity and specificity were evaluated. The established ECLIA for Flu B antigen in this study was specific, sensitive and precise. It may provide a convenient and rapid technique for clinical detection of Flu B.
