Reversal effect of tetrandrine in combination with droloxifene on cell line K562/A02 and its correlation with inducted apoptosis.
- Author:
Yun ZHOU
1
;
Bao-An CHEN
;
Ying DONG
;
Peng ZHANG
;
Jian CHENG
;
Ming SHENG
;
Ting WANG
;
Feng GAO
Author Information
1. Department of Hematology, Zhongda Hospital, Medical College of Southeast University, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Alkaloids;
administration & dosage;
Apoptosis;
drug effects;
Benzylisoquinolines;
administration & dosage;
Drug Resistance, Multiple;
Drug Resistance, Neoplasm;
drug effects;
Drug Therapy, Combination;
Flow Cytometry;
Humans;
K562 Cells;
Tamoxifen;
administration & dosage;
analogs & derivatives
- From:
Journal of Experimental Hematology
2004;12(3):321-323
- CountryChina
- Language:Chinese
-
Abstract:
To study the correlation of the reversal effect of tetrandrine (Tet) in combination with droloxifene (DRL) on multidrug resistant cell line K562/A02 and induction apoptosis, the apoptosis of K562 cells and K562/A02 cells after the treatment of Tet and DRL alone or their combination was detected by flow cytometry (FCM). The results showed 0.62 microg/ml Tet and 1.94 microg/ml DRL alone could induce apoptosis of K562 cells in time-dependent manner after culture for 48 hours. Neither Tet at 0.62 microg/ml nor DRL at 1.94 microg/ml alone could induce apoptosis of K562/A02 cells, but in the combination of them two induced apoptosis of few K562/A02 cells in culture for 72 hours. In conclusion, the mechanism of Tet and DRL reversing multidrug resistance has no correlation with the apoptosis of K562/A02 cells.
