Inhibitory effect of siRNA on bcr-abl gene expression in K562 cell line.
- Author:
Lei JIANG
1
;
Jian-Bo WU
;
Kang YU
;
Wu-Hua NI
Author Information
1. Institute of Medical Sciences, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. jianglei0299@hotmail.com
- Publication Type:Journal Article
- MeSH:
Dose-Response Relationship, Drug;
Genes, abl;
Humans;
K562 Cells;
metabolism;
RNA, Messenger;
analysis;
RNA, Small Interfering;
pharmacology;
Transfection
- From:
Journal of Experimental Hematology
2004;12(3):332-334
- CountryChina
- Language:Chinese
-
Abstract:
To explore a new way to treat CML, inhibitory effect of small interfering RNA (SiRNA) on bcr-abl fusion gene expression of K562 cell line was studied. SiRNA for bcr-abl gene was designed and transfected into K562 cells, bcr-abl gene expression was tested by RT-PCR. The results showed that bcr-abl gene expression was inhibited by using siRNA in dose-dependent manner and reduced to 19.9% and 26.6% of the control at 24 and 48 hours after transfection with 0.2 micro g siRNA respectively. K562 cells proliferation was suppressed finally, but bcr-abl gene expression restored at 72 hours. In conclusion, anti-bcr-abl siRNA can effectively inhibit bcr-abl gene expression of K562 cell line.
