Effect of arsenic trioxide on telomerase and telomerase reverse transcriptase in KM3 cell line.
- Author:
Zhen-Xing GUO
1
;
Jie JIN
Author Information
1. Department of Hematology, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Arsenicals;
pharmacology;
Cell Cycle;
drug effects;
Cell Line, Tumor;
DNA-Binding Proteins;
Humans;
Multiple Myeloma;
drug therapy;
enzymology;
pathology;
Oxides;
pharmacology;
RNA, Messenger;
analysis;
Telomerase;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2004;12(3):346-349
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effects of arsenic trioxide on multiple myeloma (MM) cell line KM(3) and its possible mechanism, cell viability was counted by trypan-blue exclusion, apoptosis was detected by morphology and DNA ladder; cell cycle was assayed by flow cytometry (FCM), telomerase activity was determined by semi-quantitative telomeric repeat amplification protocol (TRAP)-reverse transcription polymerase chain reaction (RT-PCR)-enzyme linked immunosorbent assay (ELISA), while the expression of hTERT mRNA in transcriptional level was measured by using RT-PCR. The results showed that arsenic trioxide inhibited the growth and viability of KM(3) cell and induced apoptosis; cell cycle was arrested in G(2) phase; arsenic trioxide could inhibit telomerase activity, which consisted with the downtrend of hTERT mRNA expression. In conclusion, down-regulation of telomerase activity and hTERT may play an important role in the apoptosis of MM cell line KM(3) induced by arsenic trioxide.
