Assessment on effect of short-term cryopreservation of cord blood hematopoietic cells.
- Author:
Ji HE
1
;
Jin-Hui LIU
;
Kan JIANG
;
Fa-Ming ZHU
;
Li-Xing YAN
Author Information
1. Blood Centre of Zhejiang Province, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Cell Survival;
Cryopreservation;
Fetal Blood;
cytology;
Hematopoietic Stem Cells;
physiology;
Humans
- From:
Journal of Experimental Hematology
2004;12(3):375-377
- CountryChina
- Language:Chinese
-
Abstract:
To study the effects of short-term cryopreservation of cord blood hematopoietic cells in liquid nitrogen, the viability and function of cord blood hematopoietic cells were investigated by using each of 8 samples cryopreserved for six months, one and two years after thawing respectively. Nucleated cells (NC) were detected by blood cell analyzer. CD34+ cells were analyzed by flow cytometry, CFU-GM were cultured and detected in vitro, the survival rate was determined by trypan blue staining. The results showed that the differences of recovery rate of NC, CD34+, CFU-GM were nonsignificant at three different cryopreserved times. In conclusion, the short-term storage in liquid nitrogen showed a good effect on cord blood hematopoietic cell without any significant change of activities and number of the cryopreserved hematopoietic cells.
